From the "sissy" to the killer to the wisecracking sidekick, gay and lesbian characters have long been featured on the silver screen. Here is a sampling of mainstream movies released over the last several decades with these themes: Note how the roles have changed over the years.
Suddenly, Last Summer (1959): Hollywood often resorted to a game of hide and seek when it came to the sexual identity of Tennessee Williams' characters (see the 1958 Elizabeth Taylor-Paul Newman version of Cat on a Hot Tin Roof). Here, an immoral gay man, whose face is never shown, is killed in front of his cousin (Taylor) during a European vacation.
The Children's Hour (1961): Shirley MacLaine and Audrey Hepburn star in this film version of the Lillian Hellman play, a depressing and tragic drama about the fallout from a child's rumor that two teachers are lesbian lovers.
The Detective (1968): Frank Sinatra steps into the shoes of a detective investigating the murder of a gay man in New York. Considered sensational and daring at the time, the film took audiences into gay bars and lingered at seedy cruising spots.
http://www.fsunews.com/vnews/display.v/ART/2006/01/09/43c17eef51141An interesting list of films with gay themes is included in the article.